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anti tra 1 81 primary antibody  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti tra 1 81 primary antibody
    Anti Tra 1 81 Primary Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 100 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+tra+1+81+primary+antibody/TRA-1-81+(TRA-1-81)+Mouse+mAb/pmc11625219-105-105-109
    Average 94 stars, based on 100 article reviews
    anti tra 1 81 primary antibody - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Methylation:

    Article Title: Engineering aortic valves via transdifferentiating fibroblasts into valvular endothelial cells without using viruses or iPS cells
    Article Snippet: .. For histone methylation analysis, cells were fixed with 4 % paraformaldehyde at 4 °C for 30min, then permeabilized by 0.5 % Triton X-100 at room temperature for 15min; after that the cells were incubated with anti-H3K9me3 primary antibody (1:50, Cell signaling, rabbit monoclonal, #13969), anti-H3K27me3 primary antibody (1:200, Cell signaling, rabbit monoclonal, #9733), anti-H3K4me3 primary antibody (1:1000, Cell signaling, rabbit monoclonal, #9751) at 4 °C for 1.5 h, anti-Rabbit secondary antibody(Invitrogen, donkey polyclonal, 1:200, A-21207) at 4 °C for 1 h. For TRA-1-81 and SSEA1 analysis of iPSCs, cells were fixed with 4 % paraformaldehyde at 4 °C for 30 min and then incubated with anti-TRA-1-81 primary antibody (1:1600, Cell signaling, mouse monoclonal, #4745) and anti-SSEA1 primary antibody (1:1600, Cell signaling, mouse monoclonal, #4744) at 4 °C for 1.5 h, anti-mouse secondary antibody (Invitrogen, goat polyclonal, 1:200, A-11001) at 4 °C for 1 h. Isotype-matched antibody served as negative control. ..

    Incubation:

    Article Title: Engineering aortic valves via transdifferentiating fibroblasts into valvular endothelial cells without using viruses or iPS cells
    Article Snippet: .. For histone methylation analysis, cells were fixed with 4 % paraformaldehyde at 4 °C for 30min, then permeabilized by 0.5 % Triton X-100 at room temperature for 15min; after that the cells were incubated with anti-H3K9me3 primary antibody (1:50, Cell signaling, rabbit monoclonal, #13969), anti-H3K27me3 primary antibody (1:200, Cell signaling, rabbit monoclonal, #9733), anti-H3K4me3 primary antibody (1:1000, Cell signaling, rabbit monoclonal, #9751) at 4 °C for 1.5 h, anti-Rabbit secondary antibody(Invitrogen, donkey polyclonal, 1:200, A-21207) at 4 °C for 1 h. For TRA-1-81 and SSEA1 analysis of iPSCs, cells were fixed with 4 % paraformaldehyde at 4 °C for 30 min and then incubated with anti-TRA-1-81 primary antibody (1:1600, Cell signaling, mouse monoclonal, #4745) and anti-SSEA1 primary antibody (1:1600, Cell signaling, mouse monoclonal, #4744) at 4 °C for 1.5 h, anti-mouse secondary antibody (Invitrogen, goat polyclonal, 1:200, A-11001) at 4 °C for 1 h. Isotype-matched antibody served as negative control. ..

    Negative Control:

    Article Title: Engineering aortic valves via transdifferentiating fibroblasts into valvular endothelial cells without using viruses or iPS cells
    Article Snippet: .. For histone methylation analysis, cells were fixed with 4 % paraformaldehyde at 4 °C for 30min, then permeabilized by 0.5 % Triton X-100 at room temperature for 15min; after that the cells were incubated with anti-H3K9me3 primary antibody (1:50, Cell signaling, rabbit monoclonal, #13969), anti-H3K27me3 primary antibody (1:200, Cell signaling, rabbit monoclonal, #9733), anti-H3K4me3 primary antibody (1:1000, Cell signaling, rabbit monoclonal, #9751) at 4 °C for 1.5 h, anti-Rabbit secondary antibody(Invitrogen, donkey polyclonal, 1:200, A-21207) at 4 °C for 1 h. For TRA-1-81 and SSEA1 analysis of iPSCs, cells were fixed with 4 % paraformaldehyde at 4 °C for 30 min and then incubated with anti-TRA-1-81 primary antibody (1:1600, Cell signaling, mouse monoclonal, #4745) and anti-SSEA1 primary antibody (1:1600, Cell signaling, mouse monoclonal, #4744) at 4 °C for 1.5 h, anti-mouse secondary antibody (Invitrogen, goat polyclonal, 1:200, A-11001) at 4 °C for 1 h. Isotype-matched antibody served as negative control. ..



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